| | Searching Current Courses For Fall 2016 |
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Course: |
BIO 270
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Title: | Bio Techniques, 9-12 Teachers |
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Long Title: | Bio Techniques, 9-12 Teachers |
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Course Description: | Introduces high school teachers to Recombinant DNA Technology as it is used in biomedical research. Basic information on the structure and function of DNA as the genetic material is briefly covered before the students are guided through a series of laboratory exercises designed to give hands on experience with some of the more important techniques used in current molecular biology. This laboratory experience will prepare them to teach the same techniques to students in their high school class rooms. Techniques used include: DNA isolation, Polymerase Chain Reaction (PCR), gel electrophoresis, and bacterial transformation. |
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Min Credit: | 0 |
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Max Credit: | 12 |
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Course Notes: | Entered new course 6/15/07 s@ |
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Origin Notes: | CCA |
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STANDARD COMPETENCIES:
I. Describe the emergence of biotechnology
II. Explain the basis of biotechnology as a science
III. Describe the state of biotechnology as a science and industry
IV. Describe anticipated changes for biotechnology in the future
V. Describe career paths in biotechnology
VI. Describe the structures and explain the functions of DNA, RNA, and proteins
VII. Explain the relevant genetics of humans, bacteria, and viruses
VIII. Demonstrate an understanding of modern nucleic acid techniques and apply this learning to isolate and sequence DNA
IX. Peform electrophoretic analysis of restriction fragments and demonstrate an understanding of the results
TOPICAL OUTLINE:
I. TOPIC 1: Genetics and Biotechnology
II. Overview of Molecular Biology
1. Emergence and Commercialization of Biotechnology
2. The Basis of Recombinant DNA Technology
3. The Current Status of Biotechnology Science/Industry
4. Future Trends and Possibilities
5. Careers in biotechnology
III. Essentials of Genetics
1. Nucleic acid structure and function (DNA, RNA, protein)
2. Human, bacterial, and viral genetics
IV. TOPIC 2: Nucleic Acid Techniques
A. Electrophoretic analysis of restriction fragments
B. Restriction enzymes
C. Agarose gel electrophoresis
D. Nucleic acid detection techniques
E. Polymerase Chain Reaction
F. DNA isolation
G. Assembling PCR cocktails
H. Thermocycling parameters
I. Amplicon detection - gel electrophoresis
V. TOPIC 3: Bacterial Transformation
A. Transformation techniques
B. Competent cells
C. Chemical Transformation
D. Antibiotic Selection
E. Modification of Phenotype
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