STANDARD COMPETENCIES:

 

 I.     Demonstrate an understanding of the basis of molecular biology as a science and as an industry.

 II.     Demonstrate an understanding of genetic and immunological principles that are basic to biotechnology.

 III.    Demonstrate an understanding of modern nucleic acid techniques and apply this learning to isolate and sequence DNA.

 IV.    Perform and interpret electrophoresis and restriction fragment analysis.

 V.    Demonstrate an understanding of the principles of PCR and to perform PCR amplifications.

 VI.     Perform DNA sequence analysis.

 VII.   Demonstrate knowledge and understanding of cloning and DNA analysis and peform various cloning and DNA analysis procedures.

 VIII.   Apply knowledge to transform bacteria.

 IX.     Apply knowledge to isolate and grow recombinant colonies.

 X       Isolate DNA using Sanger DiDeoxy\Dye terminator reactions.

 XI.     Apply knowledge to analyze and sequence DNA.

 TOPICAL OUTLINE:

 

 I.      Overview of Molecular Biology

 II.     Essentials of Genetics and Immunology

 III.    Electrophoretic analysis of restriction fragments

 IV.     Polymerase Chain Reaction

 V.      DNA sequencing

 VI.     Host Strain selection and molecular cloning

 VII.    Transformation techniques

 VIII.   Purification and culture of recombinant colonies

 IX.     Recombinant DNA isolation using Sanger DiDeoxy/Dye terminator reactions

 X       DNA analysis/sequencing